• Before Collection: • Ensure tube is not expired or damaged. • Choose correct citrate concentration as per the test requirement (commonly 3.2%).
• Blood Collection: • Perform venipuncture using a sterile technique. • Fill the tube up to the mark to maintain the correct blood-to-additive ratio. • Gently invert 5–6 times to mix the blood with anticoagulant thoroughly
• Sample Handling: • Do not underfill or overfill the tube, as it will affect test results. • Centrifuge the tube as needed before testing to obtain platelet-poor plasma.
• Post-Use: • Use within recommended time limits (typically within 4 hours). • Dispose of according to biohazard protocols.
• Storage: • Store at 2–25°C, away from direct sunlight and moisture. • Keep in original packaging until ready to use.

Note: Overfilling or underfilling may affect test accuracy.